AMS Labo

ZEISS

Apotome 3

Optical sectioning in fluorescence imaging for your widefield microscope

Create optical sections of your fluorescent samples – with structured Illumination, removal of out-of-focus light becomes simple and efficient, allowing you to fully focus on your research. ZEISS Apotome 3 recognizes the magnification and moves the appropriate grid into the beampath. The system then calculates your optical section from a number of images with different grid positions. It’s a totally reliable way to remove out-of-focus light, even in thicker specimens. Yet your system remains just as easy to operate as always. You get images with high contrast in the best possible resolution – simply brilliant optical sections.

  • Brilliant Optical Sections: Apotome 3 comes with three grids of different geometries, giving you the best resolution no matter which magnification you choose.
  • Free Choice of Light Source and Dyes: Apotome 3 adapts to your fluorophores and lights source. So you stay flexible when your experiments evolve in complexity and requirements.
  • More Structural Information: Your created images are improved even more by deconvolution, using a patented algorithm for structured illumination. Better recognize important structures of your examined objects.

Typical Applications

Application Task ZEISS Apotome 3 Function
Cell Culture 2D imaging
  • 2D single images
Fast imaging of a 2D image
  • Optical section available online on the monitor
Reliable detection of the marker even with strong background fluorescence
  • Automatic grid selection for optimum contrast with each objective
Combination of multiple contrast techniques
  • Any combination of fluorescence channels, brightfield, DIC and phase contrast
  • Individual configuration of each fluorescence channel as an optical section or widefield image
Live Cell Imaging Reduction of phototoxicity
  • Particularly low phototoxicity in combination with LED illumination and high sensitvie cameras like ZEISS Axiocams
Time-lapse images
  • Depending on the exposure time, up to three images per second
  • Doubling of the frame rate with “burst mode”
Vibratome Sections, Histological Samples 3D imaging
  • Automatic selection of the optimum grid for each objective
Modification of the optical section thickness
  • Grid freely selectable depending on the specimen
Penetration depth
  • Depending on the optical density of the tissue
3D reconstruction
  • Rendering of the image stack via integrated software function
  • Automatic transfer of the parameters of the individual fluorescence channels
Quantitative analysis
  • Reproducible size measurements through automatic system calibration
Whole Mounts 3D imaging
  • Multi Channel, Z Stack and Time Lapse, Deconvolution, images in raw data mode, 3D Rendering
Large image areas
  • Automatic acquisition of large sections using Tiles & Positions

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